Project Detail

PCR primer search script  

PCR primer search script is project number 549938
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Status:

Selected Providers: Redesha

Budget: $30-250

Created: 11/13/2009 at 9:28 EST

Bid Count: 6

Average Bid:
$ 170

11/15/2009 at 9:28 EST

Project Creator: btj24
Employer Rating: 10/1010/1010/1010/1010/1010/1010/1010/1010/1010/10 (1 reviews)

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Description

Program that searches PCR primers in a given dna sequence in Python
in the 5' - 3' strand and the reverse complement strand

No need for GUI
programs searches and gives the first hit as output for each strand

Primer is a piece of dna that has:
Length of 17 to 30 bases
GC-content about 50-60% (if possible)
The 3' end should have a G or C as the end base
Tm (melting temperature) between 55 and 65 ºC
Tm = 2ºC(A+T) + 4ºC (C+G) (A, T, C, G - number of respective bases)


Input:
Fasta file, or copy paste DNA sequence

output program:
Sequence primer 1 from 5' - 3':
Sequence primer 2 from 5' - 3':

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220

2 days

11-13-2009 13:45 EST

I can do the project and waiting for more details

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200

2 days

11-13-2009 10:59 EST

I can make such a script and deliver by coming Tuesday morning.

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90

1 day

11-14-2009 21:34 EST

I'm not a biology expert, so I do have a couple of extra questions and things to clarify if you pick me. I do however understand the jist of what you require, and am very experienced in Python. Hopefully we can get to work on this soon!

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150

1 day

11-15-2009 03:12 EST

(No Feedback Yet)

I can do this project, Please see my pm, thanks.

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210

10 days

11-13-2009 15:01 EST

(No Feedback Yet)

I possess 4 years of experience in development in python, mysql and java. 1 year of experience in development in PHP and c++, and 2 years Django and postgreSQL, Web Designing. My strengths are in python programming, database design, and system performance. Over the 4 years, I have developed software using Object Oriented Programming with java, delphi(basic), php(basic), django, ROR(Ruby on Rails), Ruby, python, c++(basic), and one year using Aspect Oriented Programming with python and JavaScript - Ajax and GAE(Google App Engine).

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150

10 days

11-13-2009 16:37 EST

(No Feedback Yet)

Hi, I am a graduate from Biotechnology department, IIT Madras. I have done primer search before also. Based on your detailed requirement, I can provide you exactly what you want. Kindly let me know your detailed requirements. Based on the demand, I would be more than happy to demonstrate the free mock up work in progress version. Kind Regards - Himanshu Chopra

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